a comparative investigation on zearalenone toxin production patterns during mycoprotein production by fusarium solani using carbon and nitrogen resources

نویسندگان

fereshteh jafarian

division microbiology, department of molecular and cell biology, faculty of basic sciences, lahijan branch, islamic azad university (iau), lahijan, gilan, i.r iran,سازمان اصلی تایید شده: دانشگاه آزاد اسلامی لاهیجان (islamic azad university of lahijan) arash chaichi nosrati

division microbiology, department of molecular and cell biology, faculty of basic sciences, lahijan branch, islamic azad university (iau), lahijan, gilan, i.r iran,سازمان اصلی تایید شده: دانشگاه آزاد اسلامی لاهیجان (islamic azad university of lahijan) leila modiri

division microbiology, department of molecular and cell biology, faculty of basic sciences, lahijan branch, islamic azad university (iau), lahijan, gilan, i.r iran,سازمان اصلی تایید شده: دانشگاه آزاد اسلامی لاهیجان (islamic azad university of lahijan) mohammad faezi ghasemi

division microbiology, department of molecular and cell biology, faculty of basic sciences, lahijan branch, islamic azad university (iau), lahijan, gilan, i.r iran,سازمان اصلی تایید شده: دانشگاه آزاد اسلامی لاهیجان (islamic azad university of lahijan)

چکیده

fusarium is a fungus that is commonly found as saprophytes with parasitic life on some living organisms. mycoprotein or fungus proteins with physical and chemical features are known as food in recent years. when they are involved in temperature and wet stress, release materials such as mycotoxins or fungus toxins remained in food. zearalenone and dependent compounds have distinct role in many fungus toxicity and beings especially despite acute toxicity. also the global allowed rate of zearalenone in foods is 30-1000 nano gr/ppb. the submerged culture environment to produce fungus biomass was vogel basic medium.then, the produced fungus biomasses were harvested at 4500rpm for about 20 minutes by centrifugation, washed and rinsed twice and desiccated overnight in room temperature. the dry weight biomass was measured and used in order to measure protein rate extracts for toxin estimating based on producer instructions. then, 50ml substrate was added and after 5 minutes stopper enzyme was added. finally, the zearalenone toxin amount was measured by elisa reader system. the results show that after nitrogen resource, the carbon resource play the second role in production and increase of toxin rate in mycoprotein biomass extracts. verifying the optimum submerged environment in which all optimal conditions of each steps were applied (the first priority of optimal environment include 0.25% of starch originated carbon resource and 0.25% of urea originated nitrogen resource) showed the rate of protein production was 0.642% that is have more increased about 0.207% in relation to basic submerged environment. in this environment the amount of zearalenone toxin was 0.99 ppb/gr. in the other way, when we use 2.64% of rice bran as replacement carbon resource, using 0.75% of urea in compare with 0.50% of soy bean peptone as nitrogen resources we could observe an increase of toxin rate in biomass about 0.66 %. while the amount of urea reduced to 33% and even we use 0.25% of starch instead of rice bran, the toxin in biomass reduced into 71% and reached to 0.99 ppb/gr. when we use 2.30% of meat peptone, applying 0.50% of urea in compares with 0.50% of soy bean peptone increase up to 66% of zearalenone toxin in biomass and reached to 0.77 ppb/gr.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A comparative investigation on zearalenone toxin production patterns during mycoprotein production by Fusarium solani using carbon and nitrogen resources

Fusarium is a fungus that is commonly found as saprophytes with parasitic life on some living organisms. Mycoprotein or fungus proteins with physical and chemical features are known as food in recent years. When they are involved in temperature and wet stress, release materials such as mycotoxins or fungus toxins remained in food. Zearalenone and dependent compounds have distinct role in many f...

متن کامل

In Vitro Toxin Production by Fusarium solani f. sp. piperis

Fusarium solani f. sp. piperis (teleomorph: Nectria haematococca f. sp. piperis), causal agent of root rot and stem blight on black pepper (Piper nigrum), produces secondary metabolites with toxigenic properties, capable of inducing vein discoloration in detached leaves and wilting in transpiring microcuttings. Production of F. solani f. sp. piperis (Fsp) toxic metabolites reached a peak after ...

متن کامل

T-2 toxin production by Fusarium tricinctum on solid substrate.

A method has been developed to produce and purify gram quantities of T-2 toxin [4beta, 15-diacetoxy-8alpha-(3-methylbutyryloxy)-12, 13-epoxytrichothec-9-en-3alpha-ol], a mycotoxin elaborated by a strain of Fusarium tricinctum isolated from toxic corn. After growing for 3 weeks at 15 C on 1,200 g of white corn grits, F. tricinctum NRRL 3299 elaborated at least 9.0 g of T-2 toxin, and 2.3 g of cr...

متن کامل

Optimization for the Production of Deoxynivalenol and Zearalenone by Fusarium graminearum Using Response Surface Methodology

Fusarium mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) are the mostcommon contaminants in cereals worldwide, causing a wide range of adverse health effects onanimals and humans. Many environmental factors can affect the production of these mycotoxins.Here, we have used response surface methodology (RSM) to optimize the Fusarium graminearumstrain 29 culture conditions for maximal toxin p...

متن کامل

Optimization of Growth and Bioactive Metabolite Production: Fusarium Solani

The present study aimed to evaluate optimize the growth and antibacterial activity of endophytic fungi isolated from Tylophora indica. Based on the morphological and molecular characteristic, the isolate was identified as Fusarium solani. Modified liquid medium (M2D) was used as a basal medium for growth and antibacterial activity. The growth and metabolite production were optimized with 4% dex...

متن کامل

Toxin production by Fusarium species from sugar beets and natural occurrence of zearalenone in beets and beet fibers.

Fifty-five Fusarium isolates belonging to nine species were collected from fungus-invaded tissue of stored sugar beets and identified as F. acuminatum (11 isolates), F. avenaceum (1 isolate), F. culmorum (1 isolate), F. equiseti (23 isolates), F. graminearum (4 isolates), F. oxysporum (1 isolate), F. solani (4 isolates), F. sporotrichioides (7 isolates), and F. subglutinans (2 isolates). All is...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of molecular and clinical microbiology

جلد ۳، شماره ۱، صفحات ۲۴۸-۲۵۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023